ABSTRACT
1, 2-Dimethylhydrazine [DMH] enhances lipid peroxidation rate by tumor mitochondria than normal tissue counterpart and causes many disorders in antioxidant system in liver. It also increases the level of enzymes that metabolize toxin in liver and colon. The aim of this study was to evaluate the alteration of liver and its enzymes after DMH injection and evaluate protective effect of cell wall and cytoplasmic fractions of Saccharomyces cereviseae enriched with selenium [Se] on these tissues. Forty eight female rats were prepared and acclimatized to the laboratory conditions for two weeks, and all animals received 1, 2- dimethyl hydrazine chloride [40 mg/kg body weight] twice a week for 4 weeks except healthy control. At first colon carcinoma [aberrant crypt foci] confirmed by light microscope. Then the changes resulting from injection of DMH on liver of animals in initial and advanced stages of colon cancer were examined. In addition, the protective effect of cell wall and cytoplasmic fractions of Selenium-enriched S. cerevisiae were investigated in two phases. First phase in initial stage and second phase in advanced stage of colon cancer were performed respectively. Forty weeks following the first DMH injection, all survived animals were sacrificed. Then, colon and liver removed and exsanguinated by heart puncture. For measuring the levels of enzymes [AST, ALT, and ALP], a commercial kit [Parsazmoon, Iran] and an autoanalyzer [BT 3000 Pluse, Italy] were used. The results showed that subcutaneous injection of DMH increased the ALT, AST, and ALP levels up to 78.5, 161.38, and 275.88 U/L compared to the control, respectively. Moreover, statistical analysis in both phases of experiment revealed that the enzyme levels were decreased in the treated groups in comparison with the DMH-injected group, while the levels of these enzymes were lower in the control group. It should be concluded that administration of cell wall and cytoplasmic fraction prepared from Se-enriched S. cerevisiae could reduce the tissue damages in the livers DMH-injected rats. This beneficial effect would warrant further study on the clinical application of Se-enriched yeast
ABSTRACT
Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria [isolated from common carp intestine] on K562 and the role of cell concentration on the results of MTT [3-[4,5-Dimethylthiazol-2-yl]2,5- Diphenyl tetrazolium Bromide] test
Methods: For this purpose, bacteria were cultured in specific medium [MRS broth] at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls [500, 1000, 2000 and 4000 micro g/ml] were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique
Results: The results showed extracted cell wall from both probiotic statistically [P=0.098] have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay
Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall
ABSTRACT
This study designed to test the antibacterial potency of enrofloxacin [ENR] and essential oils from Zataria multiflora Boiss [ZEO] and Trachyspermum copticum [TEO] on Aeromonas hydrophila. The antibacterial potency of test compounds was determined by several methods including the inhibition zone diameter determination, microbroth dilution method and colorimetric method of MTT. The cytotoxicity of test substances was assessed on Chinook salmon [Oncorhynchus tshawytscha] embryo [CHSE-214] cells. Results showed that ENR and tested essential oils exert antibacterial effect against A. hydrophila. Moreover, ENR exerted the most potent antibacterial effect with MIC values of 62.5 ng/ml. The natural compounds of ZEO and TEO also showed antibacterial effects with rather high MIC values of 0.315 mg/ml, and 1.25 mg/ml, respectively. None of the tested substances showed toxicity on CHSE-24 cells. It is concluded that ZEO and TEO could be applied to prevent from A. hydrophila infection. Moreover, data also suggest that MTT method could be both cost- and time-effective and accurate method of MIC determination